OrangerV1, France, Analysis, bibRecord, 000198

Additional copies of virG from pTiBo542 provide a super-transformation ability to Agrobacterium tumefaciens in citrus

Identifieur interne : 000198 ( France/Analysis ); précédent : 000197; suivant : 000199

Additional copies of virG from pTiBo542 provide a super-transformation ability to Agrobacterium tumefaciens in citrus

Auteurs : Riadh Ghorbel [Espagne] ; Stefano La-Malfa [Espagne] ; Maria M. Lopez [Espagne] ; Annik Petit [France] ; Luis Navarro [Espagne] ; Leandro Pena [Espagne]

Source :

Physiological and molecular plant pathology [ 0885-5765 ] ; 2001.

RBID : Pascal:01-0430903

Descripteurs français

Pascal (Inist)
- Transformation génétique, Aptitude, Nombre copie, Virulence, Gène, Plasmide Ti, Microorganisme recombinant, Plante transgénique, Souche, Agrobacterium tumefaciens, Citrus, DNA, Etude expérimentale, Gène virG, Plasmide pTiBo542, Souche A281, Souche C58, Souche C58(pMP90)/p35SGUSINT, Citrus aurantifolia, Citrus aurantium, Citrus limon, Citrus reshni, Citrus sinensis Poncirus trifoliata, Souche désarmée.
Wicri :
- topic : Plante transgénique.

English descriptors

KwdEn :
- Ability, Agrobacterium tumefaciens, Citrus, Copy number, DNA, Experimental study, Gene, Genetic transformation, Recombinant microorganism, Strain, Ti Plasmid, Transgenic plant, Virulence, disarmed strain.

Abstract

Agrobacterium tumefaciens strain A281 carrying pTiBo542 was described as able to induce large and early-appearing tumours on a wider range of plants than other Agrobacterium strains. Here we show a strict correlation between the supervirulence of A281 and super-transformation ability of its disarmed derivative EHA105/p35SGUSINT. This strain produced much higher transformation than the disarmed derivative of the common strain C58, called C58(pMP90)/p35SGUSINT. Transformation frequency increased from six to 22-fold, depending on the citrus genotype. This increase permitted to regenerate for the first time, whole transgenic plants from lemon, alemow and Cleopatra mandarin. Since the chromosomal background from C58 as well as the T-DNA from p35SGUSINT are identical for both bacterial strains, the super-transformation ability of EHA105/p35SGUSINT in citrus may be attributed to the vir region of pTiBo542. In addition, introduction of extra copies of virG from pTiBo542 into either EHA105/ p35SGUSINT or C58(pMP90)/p35SGUSINT further increased transformation, demonstrating the importance of vir induction, and mainly virG activation, in the super-transformation ability of this Ti plasmid in citrus.

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000875
to stream PascalFrancis, to step Curation: 000129
to stream PascalFrancis, to step Checkpoint: 000912
to stream Main, to step Merge: 003068
to stream Main, to step Curation: 002E71
to stream Main, to step Exploration: 002E71
to stream France, to step Extraction: 000198

Links to Exploration step

Pascal:01-0430903

Le document en format XML

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<author><name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name>
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<author><name sortKey="Pena, Leandro" sort="Pena, Leandro" uniqKey="Pena L" first="Leandro" last="Pena">Leandro Pena</name>
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<series><title level="j" type="main">Physiological and molecular plant pathology</title>
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</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ability</term>
<term>Agrobacterium tumefaciens</term>
<term>Citrus</term>
<term>Copy number</term>
<term>DNA</term>
<term>Experimental study</term>
<term>Gene</term>
<term>Genetic transformation</term>
<term>Recombinant microorganism</term>
<term>Strain</term>
<term>Ti Plasmid</term>
<term>Transgenic plant</term>
<term>Virulence</term>
<term>disarmed strain</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Transformation génétique</term>
<term>Aptitude</term>
<term>Nombre copie</term>
<term>Virulence</term>
<term>Gène</term>
<term>Plasmide Ti</term>
<term>Microorganisme recombinant</term>
<term>Plante transgénique</term>
<term>Souche</term>
<term>Agrobacterium tumefaciens</term>
<term>Citrus</term>
<term>DNA</term>
<term>Etude expérimentale</term>
<term>Gène virG</term>
<term>Plasmide pTiBo542</term>
<term>Souche A281</term>
<term>Souche C58</term>
<term>Souche C58(pMP90)/p35SGUSINT</term>
<term>Citrus aurantifolia</term>
<term>Citrus aurantium</term>
<term>Citrus limon</term>
<term>Citrus reshni</term>
<term>Citrus sinensis  Poncirus trifoliata</term>
<term>Souche désarmée</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Plante transgénique</term>
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<front><div type="abstract" xml:lang="en">Agrobacterium tumefaciens strain A281 carrying pTiBo542 was described as able to induce large and early-appearing tumours on a wider range of plants than other Agrobacterium strains. Here we show a strict correlation between the supervirulence of A281 and super-transformation ability of its disarmed derivative EHA105/p35SGUSINT. This strain produced much higher transformation than the disarmed derivative of the common strain C58, called C58(pMP90)/p35SGUSINT. Transformation frequency increased from six to 22-fold, depending on the citrus genotype. This increase permitted to regenerate for the first time, whole transgenic plants from lemon, alemow and Cleopatra mandarin. Since the chromosomal background from C58 as well as the T-DNA from p35SGUSINT are identical for both bacterial strains, the super-transformation ability of EHA105/p35SGUSINT in citrus may be attributed to the vir region of pTiBo542. In addition, introduction of extra copies of virG from pTiBo542 into either EHA105/ p35SGUSINT or C58(pMP90)/p35SGUSINT further increased transformation, demonstrating the importance of vir induction, and mainly virG activation, in the super-transformation ability of this Ti plasmid in citrus.</div>
</front>
</TEI>
<affiliations><list><country><li>Espagne</li>
<li>France</li>
</country>
<region><li>Île-de-France</li>
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<settlement><li>Gif-Sur-Yvette</li>
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<tree><country name="Espagne"><noRegion><name sortKey="Ghorbel, Riadh" sort="Ghorbel, Riadh" uniqKey="Ghorbel R" first="Riadh" last="Ghorbel">Riadh Ghorbel</name>
</noRegion>
<name sortKey="La Malfa, Stefano" sort="La Malfa, Stefano" uniqKey="La Malfa S" first="Stefano" last="La-Malfa">Stefano La-Malfa</name>
<name sortKey="Lopez, Maria M" sort="Lopez, Maria M" uniqKey="Lopez M" first="Maria M." last="Lopez">Maria M. Lopez</name>
<name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name>
<name sortKey="Pena, Leandro" sort="Pena, Leandro" uniqKey="Pena L" first="Leandro" last="Pena">Leandro Pena</name>
</country>
<country name="France"><region name="Île-de-France"><name sortKey="Petit, Annik" sort="Petit, Annik" uniqKey="Petit A" first="Annik" last="Petit">Annik Petit</name>
</region>
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</record>

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Additional copies of virG from pTiBo542 provide a super-transformation ability to Agrobacterium tumefaciens in citrus

Additional copies of virG from pTiBo542 provide a super-transformation ability to Agrobacterium tumefaciens in citrus

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri